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  • Title: [Delta pH-induced transport of Ca 2+ into smooth muscle plasma membrane vesicle fraction].
    Author: Kosterin SA, Fomin VP, Chervonenko IB, Shinlova OP.
    Journal: Biokhimiia; 1990 Jan; 55(1):73-9. PubMed ID: 2160842.
    Abstract:
    Using the fluorescent dye acridine orange, the feasibility of formation in myometrium sarcolemma of closed inside-out oriented vesicles and of a proton gradient created by the pH-jump method and stable in time, was demonstrated. At the initial value of delta pH = 2, the characteristic time of the gradient dissipation providing for the pH change by one unity is 4 to 5 minutes. The proton gradient oriented from the intravesicular space to the environment stimulated the Ca2+ influx into the vesicles. The transmembrane gradient of H+ with the inside-out oriented sarcolemmal vesicles prevents the Ca2+ influx. It is concluded that plasma membranes of smooth muscle cells contain alongside with the ATP- and Na(+)-dependent Ca2+ transport systems also a mechanism of the delta pH-induced transport of this bivalent cation.
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