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  • Title: Microsatellite markers in the traditional Chinese medicinal herb Gynostemma pentaphyllum (Cucurbitaceae).
    Author: Liao H, Zhao Y, Zhou Y, Wang Y, Wang X, Lu F, Song Z.
    Journal: Am J Bot; 2011 Mar; 98(3):e61-3. PubMed ID: 21613126.
    Abstract:
    PREMISE OF THE STUDY: Microsatellite primers were developed for Gynostemma pentaphyllum, a traditional Chinese medicinal herb, to investigate its population genetic diversity. METHODS AND RESULTS: Using the Microsatellite Sequence Enrichment protocol, 14 polymorphic primers sets were identified in four Chinese Gynostemma pentaphyllum populations. The primers amplified di- and trinucleotide repeats with 1-6 alleles per locus, and the observed heterozygosities ranged from 0.000 to 1.000 per population. CONCLUSIONS: These markers will facilitate further studies on genetic structure and gene flow of Gynostemma pentaphyllum populations.
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