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  • Title: An enzyme-linked immunosorbent assay for detection of bovine leukaemia virus p24 antibody in cattle.
    Author: Molloy JB, Walker PJ, Baldock FC, Rodwell BJ, Cowley JA.
    Journal: J Virol Methods; 1990 Apr; 28(1):47-57. PubMed ID: 2161419.
    Abstract:
    An ELISA for detecting antibody to the bovine leukaemia virus (BLV) core protein p24 is described. The test uses p24 antigen purified from concentrated cell culture supernate by lectin-affinity chromatography and gel filtration. The sensitivity and specificity of the p24-ELISA for diagnosing BLV infection relative to the gp51 agar gel immunodiffusion test, were 98.1 and 96.7%, respectively. In the event of widespread use of gp51 based vaccines, the p24-ELISA should differentiate effectively between naturally infected and vaccinated animals.
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