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Title: A self assembled monolayer based microfluidic sensor for urea detection. Author: Srivastava S, Solanki PR, Kaushik A, Ali MA, Srivastava A, Malhotra BD. Journal: Nanoscale; 2011 Jul; 3(7):2971-7. PubMed ID: 21614348. Abstract: Urease (Urs) and glutamate dehydrogenase (GLDH) have been covalently co-immobilized onto a self-assembled monolayer (SAM) comprising of 10-carboxy-1-decanthiol (CDT) via EDC-NHS chemistry deposited onto one of the two patterned gold (Au) electrodes for estimation of urea using poly(dimethylsiloxane) based microfluidic channels (2 cm × 200 μm × 200 μm). The CDT/Au and Urs-GLDH/CDT/Au electrodes have been characterized using Fourier transform infrared (FTIR) spectroscopy, contact angle (CA), atomic force microscopy (AFM) and electrochemical cyclic voltammetry (CV) techniques. The electrochemical response measurement of a Urs-GLDH/CDT/Au bioelectrode obtained as a function of urea concentration using CV yield linearity as 10 to 100 mg dl(-1), detection limit as 9 mg dl(-1) and high sensitivity as 7.5 μA mM(-1) cm(-2).[Abstract] [Full Text] [Related] [New Search]