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  • Title: Electrophysiological study of inner medullary collecting duct of hamsters.
    Author: Imai M, Yoshitomi K.
    Journal: Pflugers Arch; 1990 Apr; 416(1-2):180-8. PubMed ID: 2162031.
    Abstract:
    The electrophysiological properties of the hamster mid-inner medullary collecting duct (IMCD2) cells were examined in isolated and perfused preparations by intracellular impalement with conventional 1 mol/l KCl microelectrodes and cable analysis. The transmural voltage (VT) was not different from 0 mV, while the basolateral transmembrane voltage (VB) was -81.7 +/- 0.91 mV (n = 221). The transmural resistance (RT) was 109 omega cm2, indicating that the IMCD2 is composed of tight epithelia. The fractional apical membrane resistance (fRA) was 0.98 +/- 0.003 (n = 10). Abrupt changes in the luminal concentration of Na+, K+ or Cl- did not alter the apical membrane voltage (VA) or VT, and neither 2 mmol/l Ba2+ nor 10 mumol/l amiloride in the lumen affected VA and VT. Moreover, pretreatment of hamsters with deoxycorticosterone acetate (5 mg/kg, s.c.) for 10-14 days caused only a very small change in VT in the negative direction. Amiloride in the lumen increased RT and increased the voltage divider ratio very slightly. However, an abrupt increase in K+ concentration in the bath from 5 mmol/l to 50 mmol/l or addition of 2 mmol/l Ba2+ to the bath depolarized the basolateral membrane by 39 mV and 29 mV, respectively. In the presence of 2 mmol/l Ba2+ in the bath, a reduction of HCO3- concentration from 25 mmol/l to 2.5 mmol/l depolarized VB by 20.4 mV. No Cl- conductance was demonstrated in the basolateral membrane.(ABSTRACT TRUNCATED AT 250 WORDS)
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