These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: [Formation of interdisks from the P-element DNA material in Drosophila polytene chromosomes].
    Author: Semeshin VF, Demakov SA, Peres Alonso M, Zhimulev IF.
    Journal: Genetika; 1990 Mar; 26(3):448-56. PubMed ID: 2162310.
    Abstract:
    Electron microscopical (EM) analysis of regions of Drosophila melanogaster polytene chromosomes containing a matrial of the P element was carried out. In one of the stocks analysed the DNA fragment of 54 kb in length was inserted in the genome by the P element-mediated transformation. This fragment consists of four tandemly repeated copies of the rosy gene and each of them has P-DNAs at the terminal sites. In polytene chromosomes the fragment forms a complex of loose chromatin, where, nevertheless, it is possible to distinguish four bands. EM analysis of two regions with the P element in other stock reveals lengthening of interbands, as compared with the same interbands in the initial stock without the P element. In the third of the stocks analysed the P element was inserted in the region of vermilion gene which is situated in the large single band, 10A1-2 of the X-chromosome. In this case, the splitting of the band into two unequal parts and formation of a new interband was observed. Data obtained show the possibility of interband formation from DNA material of the P element, which is probably connected with its transcriptional activity.
    [Abstract] [Full Text] [Related] [New Search]