These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Ultracytochemical characteristic of ouabain-sensitive, potassium-dependent p-nitrophenylphosphatase (Na(+)-K(+)-ATPase) of rat myocardium.
    Author: Kiselyova AP, Zinchuk VS.
    Journal: Acta Histochem; 1990; 88(1):1-9. PubMed ID: 2162617.
    Abstract:
    The ultracytochemical localization of ouabain-sensitive, potassium-dependent p-nitrophenylphosphatase within a rat heart has been studied. Our cytochemical procedure for the detection of enzyme activity is based on an incubation medium consisting of tris-maleate buffer, p-nitrophenylphosphate (p-NPP) as a substrate, lead nitrate as a trapping agent, MgCl2, KCl, levamisole, final pH = 7.3. The physiological pH of the incubation medium highly increases the authenticity of the enzymatic reaction and is the main advantage of the technique. Postincubation processing of specimens by 0.1 mol/l tris-maleate buffer pH = 6.0 confirms the specificity of the reaction and removes nonspecific precipitation.
    [Abstract] [Full Text] [Related] [New Search]