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  • Title: Deuterons cannot replace protons in active transport processes in yeast.
    Author: Kotyk A, Dvoráková M, Koryta J.
    Journal: FEBS Lett; 1990 May 21; 264(2):203-5. PubMed ID: 2162783.
    Abstract:
    Replacement of ordinary water with heavy water causes a sharp reduction of the rates of both primary hydrogen ion transport (at the plasma membrane ATPase) and secondary symports (H(+)-associated transports of sugars and amino acids) in several species of yeast. At the same time, the hydrolytic activity of the ATPase is affected only very little. Likewise, the membrane potential, the delta pH and, correspondingly, the accumulation ratios of the various symported solutes are altered much less. This serves as evidence that H+ or H3O+ ions are direct participants in the various active transports of nutrients in yeast.
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