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  • Title: Scale-up study of oxalic acid pretreatment of agricultural lignocellulosic biomass for the production of bioethanol.
    Author: Lee JW, Houtman CJ, Kim HY, Choi IG, Jeffries TW.
    Journal: Bioresour Technol; 2011 Aug; 102(16):7451-6. PubMed ID: 21632241.
    Abstract:
    Building on our laboratory-scale optimization, oxalic acid was used to pretreat corncobs on the pilot-scale. The hydrolysate obtained after washing the pretreated biomass contained 32.55g/l of xylose, 2.74g/l of glucose and low concentrations of inhibitors. Ethanol production, using Scheffersomyces stipitis, from this hydrolysate was 10.3g/l, which approached the predicted value of 11.9g/l. Diafiltration using a membrane system effectively reduced acetic acid in the hydrolysate, which increased the fermentation rate. The hemicellulose content of the recovered solids decreased from 27.86% before pretreatment to only 6.76% after pretreatment. Most of the cellulose remained in the pretreated biomass. The highest ethanol production after simultaneous saccharification and fermentation (SSF) of washed biomass with S. stipitis was 21.1g/l.
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