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  • Title: Molecular recognition force spectroscopy of a specific lectin-carbohydrate interaction at single-molecule level.
    Author: Li Y, Qiao H, Xing C, Zhang J, Wang LX, Wang H, Zhang B, Tang J.
    Journal: J Struct Biol; 2011 Oct; 176(1):46-51. PubMed ID: 21640831.
    Abstract:
    Carbohydrates are involved in many essential biological recognition processes in physiological and pathological states. Thus, it is important to understand the mechanism of protein-carbohydrate interactions at molecular level. In the present study, molecular recognition force spectroscopy was applied to investigate the interactions between RCA₁₂₀, a lectin from Ricinus communis, and galactose (Gal) and asialofetuin (ASF) at the single-molecule level. RCA₁₂₀ coupled to the AFM tip could specifically recognize Gal and ASF, respectively. The unbinding forces of RCA₁₂₀-Gal and RCA₁₂₀-ASF increase linearly with the logarithm of loading rate. The results reveal that the binding capability of RCA₁₂₀ toward Gal is weaker than that of ASF, implicating a multivalent effect in the RCA₁₂₀-ASF interaction.
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