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  • Title: Repair of single nucleotide DNA mismatches transfected into mammalian cells can occur by short-patch excision.
    Author: Heywood LA, Burke JF.
    Journal: Mutat Res; 1990 Jul; 236(1):59-66. PubMed ID: 2164146.
    Abstract:
    Synthetic DNA linkers containing a single mismatched nucleotide (C:A) are repaired without bias at high efficiency when introduced into mammalian cells on a SV40 shuttle vector. From the pattern of repair in vectors containing multiple linkers, it appears that DNA synthesis following mismatch excision can replace a length of DNA as short as 40 nucleotides. Furthermore, results from the introduction of linker molecules containing combinations of single-strand nicks suggest that transient unsealed nicks do not drive the direction of mismatch repair in mammalian cells, as has previously been proposed.
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