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  • Title: Construction and structural analysis of tethered lipid bilayer containing photosynthetic antenna proteins for functional analysis.
    Author: Sumino A, Dewa T, Takeuchi T, Sugiura R, Sasaki N, Misawa N, Tero R, Urisu T, Gardiner AT, Cogdell RJ, Hashimoto H, Nango M.
    Journal: Biomacromolecules; 2011 Jul 11; 12(7):2850-8. PubMed ID: 21650465.
    Abstract:
    The construction and structural analysis of a tethered planar lipid bilayer containing bacterial photosynthetic membrane proteins, light-harvesting complex 2 (LH2), and light-harvesting core complex (LH1-RC) is described and establishes this system as an experimental platform for their functional analysis. The planar lipid bilayer containing LH2 and/or LH1-RC complexes was successfully formed on an avidin-immobilized coverglass via an avidin-biotin linkage. Atomic force microscopy (AFM) showed that a smooth continuous membrane was formed there. Lateral diffusion of these membrane proteins, observed by a fluorescence recovery after photobleaching (FRAP), is discussed in terms of the membrane architecture. Energy transfer from LH2 to LH1-RC within the tethered membrane was observed by steady-state fluorescence spectroscopy, indicating that the tethered membrane can mimic the natural situation.
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