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  • Title: Amino acid transport in membrane vesicles of Clostridium thermoautotrophicum.
    Author: Hugenholtz J, Ljungdahl LG.
    Journal: FEMS Microbiol Lett; 1990 May; 57(1-2):117-21. PubMed ID: 2165963.
    Abstract:
    A proton motive force (delta p) generated by oxidation of CO in membrane vesicles of Clostridium thermoautotrophicum drove active transport of L-alanine, glycine and L-serine. The maximum rate (Vmax) for L-alanine transport was 12 X higher at 50 degrees C than at 25 degrees C. The apparent transport constant (Kt) for L-alanine uptake was 30-40 microM and independent of the temperature. Glycine was a substrate for the L-alanine transport system as demonstrated by the competitive inhibition of L-alanine uptake by glycine (Ki = 6 microM), by the kinetics of glycine uptake (Kt = 7 microM) and by the inhibition of glycine uptake by L-alanine. The uptake kinetics of glycine was biphasic. L-Serine inhibited competitively also L-alanine and glycine transport but it was taken up by a separate transport system. The rate of amino acid transport, but not the Kt, was dependent on the value of the proton motive force.
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