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  • Title: Transcellular bicarbonate transport in rabbit gallbladder epithelium: mechanisms and effects of cyclic AMP.
    Author: Petersen KU, Wehner F, Winterhager JM.
    Journal: Pflugers Arch; 1990 May; 416(3):312-21. PubMed ID: 2166275.
    Abstract:
    HCO3 permeation through rabbit gallbladder epithelium has been investigated in vitro using voltage-clamp, pH-stat and microelectrode techniques. Mucosa-to-serosa flux of HCO3 (approximately 4.9 mumol cm-2 h-1) was dependent on luminal Na and inhibited by amiloride (1 mmol/l, luminal bath), methazolamide (0.1 mmol/l, both sides), and ouabain (30 mumol/l, serosal bath). Maximal rates of serosa-to-mucosa flux of HCO3 (approximately 2.8 mumol cm-2 h-1) required serosal Na and mucosal Cl. This flux was inhibited by ouabain, 4-acetamido-4'-isothiocyanato-stilbene-2,2'-disulfonic acid (1 mmol/l, serosal bath), and 5-nitro-2-(3-phenylpropylamino)-benzoic acid (0.1 mmol/l, luminal bath). Ineffective were methazolamide (0.1 mmol/l, both sides) and amiloride (1 mmol/l, serosal bath). 8-Br-cAMP (1 mmol/l, serosal bath) largely inhibited the absorptive and moderately stimulated the secretory flux. In tissue conductance, short-circuit current, and transmural voltage prostaglandin E1 (1 mumol/l, serosal bath) and 8-Br-cAMP caused moderate to negligible increases. No significant alterations of apical membrane potential (approximately -65 mV) and the apparent ratio of membrane resistances (Ra/Rb; approximately 1.9) were found. Cell membranes responded to luminal Cl removal mostly with a slow hyperpolarization that was mitigated by 8-Br-cAMP or, in some cases, converted into a small, transient depolarization. Our results are best explained by transcellular HCO3 transport in both directions. In secretion, basolateral HCO3 entry occurs by some form of co-transport with Na, and apical exit by Cl/HCO3 exchange. cAMP opens no major electro-diffusive pathway for apical anion efflux. In absorption, HCO3 import from the lumen into the cell is secondary to cAMP-sensitive Na/H exchange.
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