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  • Title: Trypsin-stabilized fluorescent gold nanocluster for sensitive and selective Hg2+ detection.
    Author: Kawasaki H, Yoshimura K, Hamaguchi K, Arakawa R.
    Journal: Anal Sci; 2011; 27(6):591-6. PubMed ID: 21666355.
    Abstract:
    We report on trypsin-stabilized fluorescent gold nanoclusters (Au NCs) for the sensitive and selective detection of Hg(2+) ions. The Au NCs have an average size of 1 nm and show a red emission at 645 nm. The photostable properties of the trypsin-stabilized Au NCs were examined, and their photochemical stability was found to be similar to that of CdSe quantum dots. The fluorescence was particularly quenched by Hg(2+), and therefore the Au NCs can be used as fluorescent sensors for sensitive and selective Hg(2+) detection to a detection limit of 50 ± 10 nM and the quantitative detection of Hg(2+) in wide and low concentration range of 50-600 nM.
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