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  • Title: Disseminated adenovirus (type 19) infection in a neonate. Rapid detection of the infection by immunofluorescence.
    Author: Matsuoka T, Naito T, Kubota Y, Morita Y, Takei J, Akiyama K, Hata J, Hasegawa A, Sata T, Kurata T.
    Journal: Acta Paediatr Scand; 1990 May; 79(5):568-71. PubMed ID: 2166992.
    Abstract:
    A case of fatal disseminated adenovirus infection in a neonate who suffered from severe keratoconjunctivitis and pneumonitis is reported. The diagnosis was made seven days after the onset of illness based on the detection of adenovirus antigen in the smears of the tracheal suction and conjunctival swab by immunofluorescence. Viral antigen was detected in the frozen or formalin-fixed autopsy specimens of the lungs, kidneys, spleen, liver and lymph nodes. Typical crystal arrangement of adenovirus virions was observed in the alveolar epithelial cells by electron microscopy. The isolated virus was identified to be of type 19 by a neutralization test. The IF examination using adenovirus group specific immune reagents on the smears of clinical specimens appears to be useful for rapid diagnosis of viral infections.
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