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  • Title: Facile fabrication of recyclable and active nanobiocatalyst: purification and immobilization of enzyme in one pot with Ni-NTA functionalized magnetic nanoparticle.
    Author: Wang W, Wang DI, Li Z.
    Journal: Chem Commun (Camb); 2011 Jul 28; 47(28):8115-7. PubMed ID: 21687876.
    Abstract:
    Ni-NTA functionalized iron oxide magnetic nanoparticle was synthesized and used to selectively immobilize a his-tagged enzyme from cell free extract as an active and recyclable nanobiocatalyst, where purification and immobilization of the target enzyme were accomplished in one pot.
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