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  • Title: Protocol for high-resolution electrophoresis separation of myosin heavy chain isoforms in bovine skeletal muscle.
    Author: Picard B, Barboiron C, Chadeyron D, Jurie C.
    Journal: Electrophoresis; 2011 Jul; 32(14):1804-6. PubMed ID: 21688279.
    Abstract:
    In this short communication we describe a specific protocol for SDS-PAGE separation of adult bovine myosin heavy-chain (MyHC) isoforms. The conditions defined in this protocol allow a good separation with a good reproducibility of the four MyHC isoforms (MyHC I, IIa, IIx, IIb) identified in adult skeletal muscle of this species. This procedure uses mini-gel electrophoresis system and does not involve preparation of gradient separating gels. In addition, this protocol can also be applied to the electrophoretic separation of ovine and camel MyHC isoforms.
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