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  • Title: Molecular identification of receptor for pituitary adenylate cyclase activating polypeptide.
    Author: Ohtaki T, Watanabe T, Ishibashi Y, Kitada C, Tsuda M, Gottschall PE, Arimura A, Fujino M.
    Journal: Biochem Biophys Res Commun; 1990 Sep 14; 171(2):838-44. PubMed ID: 2169736.
    Abstract:
    Pituitary adenylate cyclase activating polypeptide (PACAP) is a novel hypothalamic peptide with 38 (PACAP38) or 27 (PACAP27) amino acid residues, structurally related to vasoactive intestinal peptide (VIP). Bovine brain membrane has a PACAP specific receptor interacting with both PACAP27 and PACAP38. Affinity-labeling of the receptor with [125I]PACAP27 identified a dominant band of Mr = 60 k. The labeling density of the 60 k band decreased in the presence of unlabeled PACAP27 or PACAP38, whereas the 60 k band remained in the presence of unlabeled VIP. Binding of [125I]PACAP27 to the membrane decreased in the presence of GTP and the labeling density of the 60 k band decreased concomitantly. The results indicate that bovine brain has a specific PACAP receptor, whose apparent molecular weight is 57 k (substracting the molecular weight of [125I]PACAP27 from 60 k).
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