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  • Title: Elevated gelsolin and alpha-actin expression in a flat revertant R1 of Ha-ras oncogene-transformed NIH/3T3 cells.
    Author: Müllauer L, Fujita H, Suzuki H, Katabami M, Hitomi Y, Ogiso Y, Kuzumaki N.
    Journal: Biochem Biophys Res Commun; 1990 Sep 14; 171(2):852-9. PubMed ID: 2169737.
    Abstract:
    Expressions of gelsolin and alpha-actin have been investigated in a revertant cell line R1 and compared with the parental human activated Ha-ras oncogene-transformed NIH/3T3 (EJ-NIH/3T3), untransformed NIH/3T3 and partially revertant R2 cells. Gelsolin mRNA expression was strongest in R1 cells, intermediate in R2 and NIH/3T3 cells, and low in EJ-NIH/3T3 cells. Southern blot analysis gave neither signs of gross rearrangements nor amplification of the gelsolin gene. alpha-actin mRNA expression was restored in R1 cells to the level of NIH/3T3 cells. In R2 and EJ-NIH/3T3 cell lines, no alpha-actin transcript was detected. High gelsolin expression and restoration of alpha-actin expression may be associated with the acquirement of flat morphology and ordered cell growth pattern, which imply loss of tumorigenicity of R1 cells.
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