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  • Title: Receptor-mediated action of hepoxilin A3 releases diacylglycerol and arachidonic acid from human neutrophils.
    Author: Nigam S, Nodes S, Cichon G, Corey EJ, Pace-Asciak CR.
    Journal: Biochem Biophys Res Commun; 1990 Sep 28; 171(3):944-8. PubMed ID: 2171511.
    Abstract:
    We have previously shown that hepoxilin A3 increases the intracellular concentration of Ca+2 in human neutrophils. Herein we address the initial events of hepoxilin action on the neutrophil which precede the rise in intracellular calcium. We show that hepoxilin A3 at 10-1000 nM concentrations releases from [1-14C]-arachidonic acid labeled neutrophils diacylglycerol and unesterified arachidonic acid in a time and concentration dependent fashion. The release of arachidonic acid and diacyglycerol are receptor-mediated events which are blocked by pertussis toxin. This data shows that hepoxilin A3 stimulates phospholipases C and A2 in the cell which may be involved in the rise in cytosolic calcium. Thus, hepoxilins may represent a hitherto unrecognised class of cellular mediators.
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