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  • Title: gamma-Aminobutyric acidA receptor desensitization in mice spinal cord cultured neurons: lack of involvement of protein kinases A and C.
    Author: Ticku MK, Mehta AK.
    Journal: Mol Pharmacol; 1990 Nov; 38(5):719-24. PubMed ID: 2172778.
    Abstract:
    Desensitization of the gamma-aminobutyric acidA (GABAA) receptor was studied in cultured mammalian spinal cord neurons, using a GABA-induced 36Cl-influx assay. GABAA receptor agonists such as GABA and muscimol produced desensitization of GABAA receptor-gated Cl- channels. The ability of GABA to induce desensitization was time and concentration dependent and reversible. Involvement of protein kinase A in the desensitization phenomenon was studied by using activators of adenylate cyclase (forskolin analogs) and membrane-permeant analogs of cyclic AMP (8-bromo-cAMP and dibutyryl-cAMP). Both active forskolin and the inactive forskolin analog 1,9-dideoxyforskolin decreased GABA-induced 36Cl- influx alone, as well as when preincubated in conjunction with GABA. The effect of forskolin analogs appears to be nonspecific and unrelated to generation of cyclic AMP. GABA-induced 36Cl- influx was also inhibited directly by 8-bromo-cAMP, dibutyryl-cAMP, and cAMP. Furthermore, the protein kinase A inhibitor H-8 did not reverse the effect of cAMP analogs on the inhibition of GABA-induced 36Cl- influx. Taken together, these results suggest that cAMP analogs inhibit GABA-induced 36Cl- influx by acting via an extracellular site. The inability of the active phorbol ester to modify GABA-induced desensitization rules out the involvement of protein kinase C in the GABA receptor desensitization. These results suggest that protein kinases A and C are not involved in GABAA receptor desensitization in mouse spinal cord cultured neurons.
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