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  • Title: Inhibition of lactate dehydrogenase isoenzymes by sodium perchlorate evaluated.
    Author: Sanders GT, van der Neut E, van Straalen JP.
    Journal: Clin Chem; 1990 Nov; 36(11):1964-6. PubMed ID: 2173649.
    Abstract:
    We evaluated a method of measuring lactate dehydrogenase isoenzyme 1 (LD-1) selectively (Clin Chem 1987;33:991-2), in which all other LD isoenzymes were inhibited by adding sodium perchlorate to the reaction medium to a final concentration of 0.825 mol/L. In this study we used the different isoenzymes purified from human autopsy tissue and found that the originally published amount of inhibitor (a) increased the original LD-1 activity and (b) did not eliminate all enzyme activity of LD-2 and LD-3. Interference by LD-2 was further demonstrated. Thus we conclude that this method cannot be used for the selective determination of LD-1 because its results do not accurately reflect the original LD-1 activity.
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