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  • Title: Improvement of a Venus-based bimolecular fluorescence complementation assay to visualize bFos-bJun interaction in living cells.
    Author: Nakagawa C, Inahata K, Nishimura S, Sugimoto K.
    Journal: Biosci Biotechnol Biochem; 2011; 75(7):1399-401. PubMed ID: 21737916.
    Abstract:
    Bimolecular fluorescence complementation (BiFC) assay makes it possible to visualize protein-protein interactions in living cells. In this assay, Venus, a bright-yellow variant of green fluorescent protein, is known to produce fluorescent backgrounds due to non-specific interactions. In this study we found that the V150A mutation increased by 8.6-fold the signal-to-noise ratio in the BiFC assay of bFos-bJun interaction.
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