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  • Title: Femtomolar DNA detection by parallel colorimetric darkfield microscopy of functionalized gold nanoparticles.
    Author: Verdoold R, Gill R, Ungureanu F, Molenaar R, Kooyman RP.
    Journal: Biosens Bioelectron; 2011 Sep 15; 27(1):77-81. PubMed ID: 21752628.
    Abstract:
    We introduce a sensing platform for specific detection of DNA based on the formation of gold nanoparticles dimers on a surface. The specific coupling of a second gold nanoparticle to a surface bound nanoparticle by DNA hybridization results in a red shift of the nanoparticle plasmon peak. This shift can be detected as a color change in the darkfield image of the gold nanoparticles. Parallel detection of hundreds of gold nanoparticles with a calibrated true color camera enabled us to detect specific binding of target DNA. This enables a limit of detection below 1.0×10(-14) M without the need for a spectrometer or a scanning stage.
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