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  • Title: Comparison of a discontinuous Percoll gradient method versus a swim-up method: effects on sperm morphology and other semen parameters.
    Author: Menkveld R, Swanson RJ, Kotze TJ, Kruger TF.
    Journal: Andrologia; 1990; 22(2):152-8. PubMed ID: 2176068.
    Abstract:
    Increasing the proportion of motile and normal spermatozoa is an important objective for assisted reproductive procedures. The aim of this study was to compare the results of a Percoll and swim-up-method, especially on the sperm morphology. The Percoll technique gave a recovery rate of 81.3% of motile spermatozoa against a recovery rate of 28.98% for the swim-up technique. When the percentage of normal spermatozoa was also brought into calculation the recovery rate with the Percoll technique decreased to 62.03% and increased to 31.81% for the swim-up technique. This was due to a decrease in the resultant morphology of the Percoll technique to a median of 8.0% and an increase to 11.5% for the swim-up technique from a median of 10.5% of the original samples.
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