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  • Title: A method to extract intact and pure RNA from mycobacteria.
    Author: Akhtar S, Sarkar S, Mishra A, Sarkar D.
    Journal: Anal Biochem; 2011 Oct 15; 417(2):286-8. PubMed ID: 21767523.
    Abstract:
    We describe a high-yielding, simple, and aerosol-free protocol for the isolation of RNA from mycobacteria that does not require sophisticated instruments. The method yielded 50 μg of RNA from 10(7) cells, 50 times more than a recently reported method. Our method can extract total RNA from aerobically grown bacteria and from in vitro hypoxia-induced dormant bacilli and mycobacteria residing within infected macrophages.
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