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  • Title: Aldose reductase messenger RNA in the lens epithelium in vivo: effects of diabetes mellitus and galactosaemia.
    Author: Lightman S, Bondy C, Lightman S, Kador P.
    Journal: Clin Sci (Lond); 1990 Dec; 79(6):599-603. PubMed ID: 2176946.
    Abstract:
    1. Quantitative in situ hybridization histochemistry was used to examine the regulation of aldose reductase messenger RNA in the rat lens after the induction of diabetes mellitus or after feeding a 50% (w/w) galactose diet. 2. Although increased staining for aldose reductase in the lens epithelium has previously been observed by immunohistochemistry after 3 weeks of diabetes or after 7 days of galactose feeding, we have not been able to detect any increase in the amount of aldose reductase messenger RNA in these cells as compared with controls (113 +/- 7%, 105 +/- 9%, 100 +/- 7%, respectively) at these time points (P greater than 0.05). 3. After 15 days of galactose feeding, however, there was a significant increase of 140% (+/- 12%) in the amount of aldose reductase messenger RNA in the lens epithelial cells as compared with controls (P = less than 0.001). 4. These results demonstrate that increased availability of galactose, a high-affinity substrate for the enzyme, leads to increased aldose reductase messenger RNA, which suggests a role for aldose reductase in sugar metabolism in the lens.
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