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  • Title: HIV-1 integrase inhibitor T30177 forms a stacked dimeric G-quadruplex structure containing bulges.
    Author: Mukundan VT, Do NQ, Phan AT.
    Journal: Nucleic Acids Res; 2011 Nov 01; 39(20):8984-91. PubMed ID: 21771859.
    Abstract:
    T30177 is a G-rich oligonucleotide with the sequence (GTGGTGGGTGGGTGGGT) which inhibits the HIV-1 integrase activity at nanomolar concentrations. Here we show that this DNA sequence forms in K(+) solution a dimeric G-quadruplex structure comprising a total of six G-tetrad layers through the stacking of two propeller-type parallel-stranded G-quadruplex subunits at their 5'-end. All twelve guanines in the sequence participate in the G-tetrad formation, despite the interruption in the first G-tract by a thymine, which forms a bulge between two adjacent G-tetrads. In this work, we also propose a simple analytical approach to stoichiometry determination using concentration-dependent melting curves.
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