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  • Title: [Parvovirus B19 among blood donors from three hospitals in Santiago, Chile].
    Author: Lévican J, Torres M, Gaggero N, Corvalán R, Gaggero A.
    Journal: Rev Med Chil; 2011 Feb; 139(2):143-9. PubMed ID: 21773649.
    Abstract:
    BACKGROUND: Parvovirus B19 (B19) is associated with a wide range of diseases in humans, whose severity depends on the immunological and hematological status of the host. It is transmitted mainly through the airway but also by transfusions. AIM: To determine the B19 DNA carrier frequency in a population of volunteer blood donors from three hospitals blood banks in Santiago, Chile, and to determine the viral load in DNA positive cases. MATERIAL AND METHODS: A total of 477 serum samples were analyzed. The screening of B19 DNA was carried out by nested polymerase chain reaction (PCR) directed to the non-structural region of the virus (NS1). The viral load in positives cases was quantified by NS1 Real Time PCR. RESULTS: Parvovirus B19 was detected in four samples, rendering a frequency of 1:119. The viral loads ranged from less than 2000 to 5626 x 10(5) genome equivalents/ml. CONCLUSIONS: Parvovirus B19 was present in four of 477 blood bank blood donors from three hospitals in Santiago.
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