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  • Title: Fingerprinting of Mycoplasma gallisepticum strains isolated from multiple-age layers vaccinated with live F strain.
    Author: Kleven SH, Khan MI, Yamamoto R.
    Journal: Avian Dis; 1990; 34(4):984-90. PubMed ID: 2177979.
    Abstract:
    Mycoplasma gallisepticum (MG) isolates were obtained from three multiple-age commercial layer farms on which live F strain vaccine had been administered to each replacement flock for at least 2 years. All such isolates had restriction endonuclease DNA and sodium dodecyl sulfate-polyacrylamide gel electrophoresis protein patterns characteristic of F strain. These cultures also hybridized in dot blot assays with both the MG strain-specific and species-specific DNA probes. In contrast, the original MG isolate that came from one of the farms before vaccination began clearly was not F strain. These results suggest that continuous use of live F strain vaccine in each replacement pullet flock on multiple-age commercial layer sites will result in displacement of the original field strain of MG with the vaccine strain.
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