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  • Title: Production of 2,3-butanediol from glucose by GRAS microorganism Bacillus amyloliquefaciens.
    Author: Yang T, Rao Z, Zhang X, Lin Q, Xia H, Xu Z, Yang S.
    Journal: J Basic Microbiol; 2011 Dec; 51(6):650-8. PubMed ID: 21780143.
    Abstract:
    In the current study, a GRAS (Generally Recognized As Safe) strain of Bacillus amyloliquefaciens producing 2,3-butanediol (2,3-BD) designated as B10-127 was isolated in our lab. The strain B10-127 produced 2,3-BD effectively under the condition of 20% glucose (quality concentration), showed a high-glucose tolerance. The effects of initial glucose concentration, temperature, pH and agitation on 2,3-BD production were investigated in this work and the proper parameters were identified. Accordingly, the fed-batch culture of B10-127 in larger scales (5 l) showed a remarkable 2,3-BD producing potency. The maximum 2,3-BD concentration reached 92.3 g/l at 96 h with a 2,3-BD productivity of 0.96 g/l h. To our knowledge, the results were new records on 2,3-BD fermentation by Bacillus, which shown an excellent candidate for the microbial fermentation of 2,3-BD on an industrial scale.
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