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  • Title: Changes in the protein synthesis pattern during a nutritional shift-down transition in Saccharomyces cerevisiae.
    Author: Rodriguez F, Popolo L, Vai M, Lacanà E, Alberghina L.
    Journal: Exp Cell Res; 1990 Apr; 187(2):315-9. PubMed ID: 2180735.
    Abstract:
    In Saccharomyces cerevisiae cells (strain A364A) during a shift-down from glucose to raffinose, a rapid reduction in the rate of RNA accumulation was observed whereas the rate of protein accumulation was unaffected for at least 2 h. Following the transition the percentage of unbudded cells slightly increased and the cell volume distribution showed a newly formed subpopulation of smaller cells. To study the effects of the shift-down on the protein synthesis pattern, total [35S]-methionine pulse-labeled extracts were fractionated by high-resolution two-dimensional gel electrophoresis. The synthesis of two classes of proteins (I and II) was modulated during the transitory state of growth: one positively, the other negatively. Two polypeptides of 57 kDa showed the most dramatic increase in synthesis during the shift-down. Also a heat-shock protein (HSP 256) appeared to be positively correlated to the shift-down transition.
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