These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: High-throughput transposon mutagenesis of Corynebacterium glutamicum.
    Author: Suzuki N, Inui M, Yukawa H.
    Journal: Methods Mol Biol; 2011; 765():409-17. PubMed ID: 21815106.
    Abstract:
    Construction of gene disruption mutants and analysis of the resultant phenotypes are an important strategy to study gene function. A simple and high-throughput method developed for microorganisms combines two different types of transposons, direct genomic DNA amplification and thermal asymmetric interlaced-PCR. The considerable utility of this approach is demonstrable in Corynebacterium glutamicum, where 18,000 transposon disruptants enabled the generation of an insertion library covering nearly 80% of the organism's 2,990 ORFs.
    [Abstract] [Full Text] [Related] [New Search]