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  • Title: Increases in gene-targeting frequencies due to disruption of kueA as a ku80 homolog in citric acid-producing Aspergillus niger.
    Author: Honda Y, Kobayashi K, Kirimura K.
    Journal: Biosci Biotechnol Biochem; 2011; 75(8):1594-6. PubMed ID: 21821927.
    Abstract:
    Low efficiencies of gene targeting hamper functional genomics in industrially important strains of Aspergillus niger. To generate strains showing high gene-targeting frequencies in A. niger WU-2223L producing citric acid, disruption of kueA encoding Ku80 homolog was performed. Disruption of kueA increased gene-targeting frequencies to 70%, and had no effect on citric acid production.
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