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  • Title: Immobilization of inulinase from Kluyveromyces marxianus NRRL Y-7571 using modified sodium alginate beads.
    Author: Richetti A, Munaretto CB, Lerin LA, Batistella L, Vladimir Oliveira J, Dallago RM, Astolfi V, Luccio MD, Mazutti MA, de Oliveira D, Treichel H.
    Journal: Bioprocess Biosyst Eng; 2012 Mar; 35(3):383-8. PubMed ID: 21833621.
    Abstract:
    An experimental design was carried out to evaluate the effect of the concentrations of sodium alginate, glutaraldehyde and activated coal on the immobilization of inulinase from Kluyveromyces marxianus NRRL Y-7571. The experimental condition of 20 g/L of sodium alginate, 50 mL/L of glutaraldehyde and 30 g/L of activated coal led to the highest specific activity (2,063.5 U/mg of protein), corresponding to an enhancement of about 26 times compared to the activity of the free enzyme (79.1 U/mg of protein). The effect of pH and temperature on the immobilized enzyme activity was also evaluated, showing optimal activities at pH of 5.5 and 55 °C. The study of storage of immobilized inulinase in different temperatures showed that the extract kept its initial activity after 43 days of storage at 40 and 50 °C and after 138 days of storage either at 4 or 25 °C.
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