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  • Title: Discrimination of neutral oligosaccharides through a nanopore.
    Author: Bacri L, Oukhaled A, Hémon E, Bassafoula FB, Auvray L, Daniel R.
    Journal: Biochem Biophys Res Commun; 2011 Sep 09; 412(4):561-4. PubMed ID: 21839725.
    Abstract:
    The detection of oligosaccharides at the single-molecule level was investigated using a protein nanopore device. Neutral oligosaccharides of various molecular weights were translocated through a single α-hemolysin nanopore and their nano-transit recorded at the single-molecule level. The translocation of maltose and dextran oligosaccharides featured by 1→4 and 1→6 glycosidic bonds respectively was studied in an attempt to discriminate oligosaccharides according to their polymerization degree and glycosidic linkages. Oligosaccharides were translocated through a free diffusion regime indicating that they adopted an extended conformation during their translocation in the nanopore. The dwell time increased with molecular mass, suggesting the usefulness of nanopore as a molecular sizing device.
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