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  • Title: [Quantification of acrylamide in foods by gas chromatography-mass spectrometry coupled with pro-column derivatization].
    Author: Yang S, Zhang H, Wang J, Chen F.
    Journal: Se Pu; 2011 May; 29(5):404-8. PubMed ID: 21847973.
    Abstract:
    The quantification of acrylamide in foods was investigated by gas chromatography-mass spectrometry (GC-MS). In this method, (13)C3-acrylamide was used as an internal standard. The samples were homogenized, defatted with hexane and extracted with ethyl acetate for twice. The derivatization of acrylamide into 2-bromopropenamide (2-BPA) was done using potassium bromide/potassium bromate at 4 degrees C for 90 min. Selective ion monitoring mode was chosen to monitor objective chromatography. This method had a good linearity between 0.05-2.00 mg/kg (r2 = 0.9995), and the limits of detection and quantification were 3 microg/kg and 7 microg/kg, respectively. The recoveries of acrylamide were in the range of 62.7%-65.5%. In order to confirm this GC-MS method, the acrylamide in foods was also determined by high performance liquid chromatography coupled to quadrupole tandem mass spectrometry (HPLC-MS/MS), which had been proved in our laboratory. The concentrations of acrylamide in the samples quantified by GC-MS were slightly higher than those detected by HPLC-MS/MS. This method can be used to quantify acrylamide in normal foods.
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