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  • Title: Ethanol fermentation by nystatin-resistant strains of Saccharomyces cerevisiae.
    Author: Grunwald-Raij H, Margalith P.
    Journal: J Appl Bacteriol; 1990 Mar; 68(3):247-52. PubMed ID: 2187846.
    Abstract:
    Nystatin-resistant mutants of haploid and polyploid strains of Saccharomyces cerevisiae were isolated by plating on gradient plates with increasing nystatin concentrations (60-3000 U/ml). Some of the mutants were defective in ergosterol biosynthesis, and produced zymosterol and cholestatetraenol-like sterols. Those mutants which do not form ergosterol produce less ethanol than the parent strains. They also had lower viability during fermentation of glucose solutions (8-13% vs. 33-47%). This became more pronounced in fermentations of higher concentrations of glucose. A nystatin-resistant but ergosterol-forming mutant had a similar fermentation capacity to the parent strain.
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