These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


PUBMED FOR HANDHELDS

Search MEDLINE/PubMed


  • Title: Quantification of pork, chicken and beef by using a novel reference molecule.
    Author: Sakai Y, Kotoura S, Yano T, Kurihara T, Uchida K, Miake K, Akiyama H, Tanabe S.
    Journal: Biosci Biotechnol Biochem; 2011; 75(9):1639-43. PubMed ID: 21897035.
    Abstract:
    A standard plasmid was constructed as a novel reference molecule for use in real-time quantitative PCR assays to verify the identity of beef, pork, chicken, mutton, and horseflesh. The plasmid contained a target domain of the cytochrome b (cyt b) gene and an artificial DNA sequence. Primers CO-F and CO-R, and probe CO-P were specifically designed to detect the artificial sequence. The calculated R² values of the standard curves (10³-10⁷ copies per reaction) for the five species ranged between 0.998 and 0.999 in the quantification analysis. The constructed plasmid provides a universal method for measuring the copy number of cyt b DNA in minced meat. This method would be a useful procedure for verifying food labels.
    [Abstract] [Full Text] [Related] [New Search]