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Title: Quantification of pork, chicken and beef by using a novel reference molecule. Author: Sakai Y, Kotoura S, Yano T, Kurihara T, Uchida K, Miake K, Akiyama H, Tanabe S. Journal: Biosci Biotechnol Biochem; 2011; 75(9):1639-43. PubMed ID: 21897035. Abstract: A standard plasmid was constructed as a novel reference molecule for use in real-time quantitative PCR assays to verify the identity of beef, pork, chicken, mutton, and horseflesh. The plasmid contained a target domain of the cytochrome b (cyt b) gene and an artificial DNA sequence. Primers CO-F and CO-R, and probe CO-P were specifically designed to detect the artificial sequence. The calculated R² values of the standard curves (10³-10⁷ copies per reaction) for the five species ranged between 0.998 and 0.999 in the quantification analysis. The constructed plasmid provides a universal method for measuring the copy number of cyt b DNA in minced meat. This method would be a useful procedure for verifying food labels.[Abstract] [Full Text] [Related] [New Search]