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  • Title: Production of lipases by solid state fermentation using vegetable oil-refining wastes.
    Author: Santis-Navarro A, Gea T, Barrena R, Sánchez A.
    Journal: Bioresour Technol; 2011 Nov; 102(21):10080-4. PubMed ID: 21903382.
    Abstract:
    Lipases were produced by a microbial consortium derived from a mixture of wastewater sludges in a medium containing solid industrial wastes rich in fats, under thermophilic conditions (temperature higher than 45°C for 20 days) in 4.5-L reactors. The lipases were extracted from the solid medium using 100mM Tris-HCl, pH 8.0 and a cationic surfactant agent (cetyltrimethylammonium chloride). Different doses of surfactant and buffer were tested according to a full factorial experimental design. The extracted lipases were most active at 61-65°C and at pH 7.7-9. For the solid samples, the lipolytic activity reached up to 120,000 UA/g of dry matter. These values are considerably higher than those previously reported in literature for solid-state fermentation and highlight the possibility to work with the solid wastes as effective biocatalysts.
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