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  • Title: Effect of scriptaid, a histone deacetylase inhibitor, on the developmental competence of Handmade cloned buffalo (Bubalus bubalis) embryos.
    Author: Panda SK, George A, Saha A, Sharma R, Singh AK, Manik RS, Chauhan MS, Palta P, Singla SK.
    Journal: Theriogenology; 2012 Jan 01; 77(1):195-200. PubMed ID: 21924478.
    Abstract:
    The aim of the present study was to investigate the effect of scriptaid, a histone deacetylase inhibitor, on the developmental competence of Handmade cloned (HMC) and parthenogenetically activated (PA) buffalo embryos. Scriptaid treatment was given for 10 h immediately after reconstruction of HMC and PA embryos. Treatment of HMC embryos with 500 or 1000 nmol/L scriptaid significantly increased (P<0.01) the cleavage rate (91.3±2.8 and 91.9±2.0%, respectively) than that of controls (80.7±2.4%). Blastocyst rate was significantly higher (P<0.01) following treatment with 1000 nmol/L (54.1±5.0%) than that with 500 nmol/L scriptaid (42.6±2.9%) which, in turn, was higher (P<0.01) than that of the controls (38.0±2.6%). Also, HMC embryos treated with 500 or 1000 nmol/L scriptaid had higher (P<0.01) cell number (339.9±1.4 and 343.4±2.4, respectively) than the untreated embryos (150.7±2.0). In contrast, in zona-free PA embryos, the cleavage and blastocyst rate was significantly lower (P<0.01) following treatment with 500 (46.3±1.0 and 22.0±1.4%, respectively) or 1000 nmol/L scriptaid (46.2±1.9 and 16.6±1.3%, respectively) than that of untreated embryos (60.4±3.2 and 29.5±2.1%, respectively). Transfer of HMC embryos that had been untreated (N=13) or treated with 1000 nmol/L scriptaid (N=15) to synchronized recipients resulted in one pregnancy each. In conclusion, these results demonstrate that scriptaid treatment improves the developmental potential of HMC buffalo embryos, but compromises that of zona-free PA embryos.
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