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  • Title: Characterization of Lactobacillus isolates from fermented olives and their bacteriocin gene profiles.
    Author: Hurtado A, Ben Othman N, Chammem N, Hamdi M, Ferrer S, Reguant C, Bordons A, Rozès N.
    Journal: Food Microbiol; 2011 Dec; 28(8):1514-8. PubMed ID: 21925038.
    Abstract:
    Near one hundred isolates of Lactobacillus paraplantarum, Lactobacillus pentosus and Lactobacillus plantarum from table olives were studied. Strains were genotyped by rep-PCR. Although the technique failed to differentiate some isolates at the species level, it proved a robust and easy procedure that could be useful for distinguishing between related strains of L. paraplantarum, L. pentosus and L. plantarum from a large pool of unrelated strains of these species. A PCR-based screening revealed the presence of the plantaricin encoding genes plnA, plnB, plnC, plnD, plnE/F, plnF, plnI, plnJ, plnK, plnG and plnN in most isolates of the three species. Sequences of bacteriocin genes present in L. paraplantarum and L. pentosus were homologous to L. plantarum genes. Through a discriminating analysis of the bacteriocin gene profiles, it was possible to establish a relationship between the origin of isolation and the LAB isolates, regardless of species.
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