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  • Title: Detecting protein-protein interactions with the Split-Ubiquitin sensor.
    Author: Dünkler A, Müller J, Johnsson N.
    Journal: Methods Mol Biol; 2012; 786():115-30. PubMed ID: 21938623.
    Abstract:
    A detailed understanding of a cellular process requires the knowledge about the interactions between its protein constituents. The Split-Ubiquitin technique allows to monitor and detect interactions of very diverse proteins, including transcription factors and membrane-associated proteins. The technique is based on unique features of ubiquitin, the enzymes of the ubiquitin pathway, and the reconstitution of a native-like ubiquitin from its N- and C-terminal fragments. Using Ura3p as a reporter for the reconstitution of the ubiquitin fragments, methods are presented that enable to screen in yeast for interaction partners of a given protein with either a randomly generated expression library or a defined but more limited array of protein fusions.
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