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  • Title: The effect of IGF-1 and FSH on the in vitro development of caprine secondary follicles and on the IGF-1, IGFR-I and FSHR mRNA levels.
    Author: Brito IR, Saraiva MV, Araújo VR, Celestino JJ, Magalhães-Padilha DM, Lima IM, Van den Hurk R, Figueiredo JR, Silva JR.
    Journal: Res Vet Sci; 2012 Oct; 93(2):729-32. PubMed ID: 21963245.
    Abstract:
    This study verified the in vitro effects of IGF-1, FSH or both on caprine preantral follicle development and mRNA levels encoding IGF-1, IGFR-1 and FSHR. Secondary follicles were cultured for six days with FSH, IGF-1 or IGF-1+FSH. The results showed that IGF-1 and/or FSH addition did not influence follicular development for six days. The interaction between IGF-1 and FSH increased the mRNA levels of IGF-1 and FSHR, and FSH increased the expression of the IGFR-1 mRNA. Thus, IGF-1 and/or FSH increased IGF-1, IGFR-1 and FSHR mRNA levels in in vitro cultured caprine secondary follicles, but they did not influence their development after six days of in vitro culture.
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