These tools will no longer be maintained as of December 31, 2024. Archived website can be found here. PubMed4Hh GitHub repository can be found here. Contact NLM Customer Service if you have questions.


Pubmed for Handhelds

PUBMED FOR HANDHELDS

Search MEDLINE/PubMed

  • Title: Small-molecule ligands strongly affect the Förster resonance energy transfer between a quantum dot and a fluorescent protein.
    Author: Zhang Y, Zhang H, Hollins J, Webb ME, Zhou D.
    Journal: Phys Chem Chem Phys; 2011 Nov 21; 13(43):19427-36. PubMed ID: 21971088.
    Abstract:
    We report herein the study of Förster resonance energy transfer (FRET) between a CdSe/ZnS core/shell quantum dot (QD) capped with three different small-molecule ligands, 3-mercaptopropionic acid (MPA), glutathione (GSH), and dihydrolipoic acid (DHLA), and a hexa-histidine (His(6))-tagged fluorescent protein, mCherry (FP). The Förster radius (R(0)) and the corresponding donor-acceptor distances (r) for each of the QD-FP FRET systems were evaluated by using the Förster dipole-dipole interaction formula. Interestingly, both the FRET efficiency (E) and r were found to be strongly dependent on the capping small-molecule ligands on the QD surface, where E ≈ 85% was obtained at a FP:QD copy number of 2:1 for the MPA capped QD, while that for the DHLA capped QD was <25% under the same conditions. A molecular model was proposed to explain the possible reasons behind these observations. The dissociation constants (K(d)s) and kinetics of the self-assembled QD-FP systems were also evaluated. Results show that the QD-FP self-assembly process is fast (completes in minutes at low nM concentrations), strong (with K(d) ≈ 1 nM) and positively cooperative (with the Hill coefficient n > 1), suggesting that the QD-His(6)-tagged biomolecule self-assembly is a facile, effective approach for making compact QD-bioconjugates which may have a wide range of sensing and biomedical applications.
    [Abstract] [Full Text] [Related] [New Search]