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  • Title: [Determination of total content of ten ginsenosides in yiqifumai lyophilized injection by near infrared spectroscopy].
    Author: Han X, Li D, Zhou D, Lin R, Liu L, Ye Z.
    Journal: Zhongguo Zhong Yao Za Zhi; 2011 Jun; 36(12):1603-5. PubMed ID: 22007542.
    Abstract:
    OBJECTIVE: To determine the total content of 10 ginsenosides in Yiqifumai lyophilized injection by near infrared spectroscopy. METHOD: Sixty samples were collected and determined of the total contents of ten ginsenosides by HPLC. The optimal calibration model was established by the contents of 10 ginsenosides in fifty samples and their NIR spectroscopy using the PLS. And the contents of 10 samples were successfully predicted. RESULT: When using the pretreatment of the first derivative and MSC in the range of 4 246.8 - 4 602.2, 5 446.8 - 61 02.6 cm(-1), the best dimension was 9, and the quantitative model was accurate. The R2 was 94.2, and the RMSECV was 0.186. The RMSEP of ten samples was 0.234. CONCLUSION: This method is easy, rapaid and precise, and can be used to determine the content of 10 ginsenosides in Yiqifumai lyophilized injection.
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