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  • Title: Label-free real-time imaging in microchip free-flow electrophoresis applying high speed deep UV fluorescence scanning.
    Author: Köhler S, Nagl S, Fritzsche S, Belder D.
    Journal: Lab Chip; 2012 Feb 07; 12(3):458-63. PubMed ID: 22011722.
    Abstract:
    We report on label-free monitoring of microfluidic free-flow electrophoresis (μFFE) separations in real-time using a custom built high speed deep UV laser scanner. In combination with a novel layout realized in fused silica (FS) FFE chips the setup was successfully applied for continuous separations and detection of unlabeled analytes including native proteins by space-resolved intrinsic deep UV fluorescence scanning.
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