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  • Title: Negative-strand RNA replication by Q beta and MS2 positive-strand RNA phage replicases.
    Author: Shaklee PN.
    Journal: Virology; 1990 Sep; 178(1):340-3. PubMed ID: 2202149.
    Abstract:
    Plasmids were constructed which allowed preparation of positive- and negative-strand MS2 RNA by in vitro transcription and which expressed the MS2 phage replicase. Transcribed, positive-strand MS2 RNA was infectious to Escherichia coli spheroplasts. Transcribed, negative-strand MS2 RNA was infectious to spheroplasts containing either MS2 replicase or related bacteriophage Q beta replicase.
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