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  • Title: Differences in flow cytometry and 3H-thymidine analyses of perturbed human lymphocytes.
    Author: Pollack A, Bagwell CB, Hudson JL, Irvin GL.
    Journal: J Histochem Cytochem; 1979 Jan; 27(1):486-90. PubMed ID: 220326.
    Abstract:
    A calf thymocyte crude aqueous extract was tested for DNA synthesis inhibitory activity using phytohemagglutinin-stimulated human peripheral blood lymphocytes. Inhibition of DNA synthesis was assayed using tritiated thymidine and flow cytometry. Although the calf thymocyte crude extract inhibited tritiated thymidine incorporation by over 50%, only very slight changes in the flow cytometric analysis were observed. When dibutyryl-cyclic adenosine monophosphate was used as an inhibitor, a correlation in terms of the inhibition of tritiated thymidine to the inhibition by flow cytometry was observed.
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